rabbit antigapdh (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rabbit antigapdh
Rabbit Antigapdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antigapdh/pm41936701-483-49-51
Average 86 stars, based on 1 article reviews

rabbit antigapdh - by Bioz Stars, 2026-09

86/100 stars

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Blocking Assay:

Article Title: Reticulocalbin1-mediated regulation of calcium homeostasis in naïve T lymphoncytes.
Article Snippet: .. After blocking for 30 min, the membranes were incubated overnight at 4 ◦C with primary antibodies, anti-RCN1(abcam, EPR17163), anti-Calreticulin (Cell Signaling, #2891), anti-Calnexin (Abcam, ab22595), anti-β-Actin (Cell Signaling, #4970) and antiGAPDH (Cell Signaling, #5174). .. Following washing, the membranes were incubated with anti-rabbit IgG, HRP-linked (Cell Signaling, #7074) secondary antibodies for 1 h at room temperature.

Article Title: Generation and ophthalmological characterization of oculocutaneous albinism type 1 pig models by selection-free genome editing.
Article Snippet: .. Then, the proteins were transferred to a PVDF membrane (Millipore Corporation, Billerica, MA, USA), blocked with EveryBlot Blocking Buffer (Bio-Rad) for 10 min at room temperature, and incubated overnight at 4 °C with the following antibodies: anti-TYR (1:1000, Abcam, ab170905) and antiGAPDH (1:1000, Cell Signaling, #2118). ..

Incubation:

Article Title: IER3 Promotes Non-Small Cell Lung Cancer Malignancy by Suppressing Ferroptosis via the AKT/GSK3β/NRF2 Pathway.
Article Snippet: Non-small cell lung cancer (NSCLC) remains a lethal malignancy due to therapy resistance and recurrence.. Ferroptosis, a regulated form of cell death, is a promising strategy to overcome cancer drug resistance, yet its mechanisms remain incompletely defined.. Here, we report that Immediate Early Response 3 (IER3) is significantly upregulated in NSCLC tumors and linked to advanced stage and poor prognosis.

Transfection:

Article Title: ARMH4 accelerates aging by maintaining a positive-feedback growth signaling circuit.
Article Snippet: The next day, after washing three times with TBST, the membrane is incubated with secondary antibody at room temperature for 1 h, followed by another three washes with TBST, and visualized by ECL Western Blotting Detection Kit (Thermo Fisher, #32109). .. Specific primary antibodies included: anti-FGFR1 (1:1000, CST, #9740), anti-IGF1R (1:1000, CST, #9750), antiGAPDH (1:1000, CST, #2118), anti-FLAG (1:1000, CST, #8146), anti-HA (1:1000, CST, #3724), anti-Puromycin (1:5000, Merck-Millipore, #MABE343), anti-p-mTOR (1:1000, CST, #5536), anti-mTOR (1:1000, CST, #2983), anti-p-p70S6K (1:1000, CST, #9234), anti-p70S6K (1:1000, CST, #2708), anti-p-Akt (1:1000, CST, #4060), anti-Akt (1:1000, CST, #9272), anti-p-ERK (1:1000, CST, #4370), anti-ERK (1:1000, CST, #9102), anti-p-4E-BP1 (1:1000, CST, #2855), anti-4E-BP1 (1:1000, CST, #9644), anti-p62 (1:1000, CST, #8025), anti-LC3B (1:1000, CST, AR TI CL E IN P RE SS #3868), anti-c-Myc (1:1000, Abcam, ab32072), and anti-ARMH4 (1:500, ATLAS, #hpa001580). co-immunoprecipitation (co-IP) HEK293 cells transfected with corresponding plasmids were homogenized using the precooled lysis buffer (50 mM Tris at pH = 7.5, 150 mM NaCl, 2 mM EDTA at pH = 8.0, 1% NP-40, 5% Glycerol), thoroughly mixed by pipetting, and lysed at 4 °C with rotation for 30 min. .. The lysates were then centrifuged at 4 °C at 12,000 × g for 15 min. A small portion of the cell lysate was collected as the input, and the remaining lysate was incubated with protein A beads (Thermo fisher, #20333) for 30 min for pre-clearing.

Lysis:

Membrane:

Article Title: Target choice and exon skipping regulate CRISPR-directed gene editing of NRF2 in head/neck and esophageal cancer cells.
Article Snippet: Protein was transferred onto a 0.2 μM nitrocellulose blot using the Turboblot dry transfer system (Bio-Rad). .. The membrane was blocked in 5% milk in TBS-T for 2 h at room temperature and stained with anti-NRF2 1:1,000 (Abcam, ab62352) and antiGAPDH 1:5,000 (Cell Signaling Technology, 97166). .. Blots were washed 3× at room temperature in TBS-T and stained with secondary antibodies conjugated to HRP (horseradish peroxidase) 1:10,000 (Abcam, ab205718 or Thermo Fisher, PI31430).

Staining:

Western Blot:

Article Title: Mesothelin Promotes Acute Myeloid Leukemia Progression through LYN-dependent Signaling.
Article Snippet: For immunoprecipitations, anti-MSLN (clone MN-1) and anti-LYN polyclonal antibodies were purchased from Millipore Sigma and ThermoFisher Scientific respectively. .. For western blotting, anti-MSLN (clone D4X7M), anti-LYN (clone C13F9), antiGAPDH (clone D4C6R), anti-Vinculin (clone E1E9V) and anti-phospho (clone 197G2) and total ERK1/2 antibodies were from Cell Signaling Technologies. .. NOMO-1 cells (German Collection of Microorganisms, DSMZ) were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), 1mM/L L-glutamine, 25U/mL penicillin, and 25g/mL streptomycin.

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